Invitrogen™ Rat TCK-1 (PPBP) ELISA Kit

Catalog No.	Quantity
50-254-5329	96 Tests
50-254-5327	5 x 96 Tests
50-254-5328	10 x 96 Tests

Catalog No. 50-254-5329 Supplier Invitrogen™ Supplier No. ERPPBP

Description

ELISA

The Rat TCK-1 (PPBP) ELISA quantitates Rt TCK-1 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt TCK-1. Principle of the method The Rat TCK-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Members of the a-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a similar motif, in which the first two cysteine residues are separated by a single residue (Cys-X-Cys), and are also chemotactic for neutrophils. The platelet basic protein (PBP), a member of the a-chemokine family, resides in the a-granules of platelets and is released upon their activation. Proteolytic cleavage of the amino terminus of PBP leads to the generation of several peptides, which include mature PBP, connective tissue-activating peptide III (CTAP III, also designated low affinity platelet factor IV (LA-PF4)), b-thromboglobulin (b-TG), and neutrophil-activating peptide 2 (NAP-2). PBP and its N-truncated derivatives mediate inflammation and wound healing. Specifically, NAP-2 activates chemotaxis and degranulation in neutrophils during inflammation. The gene encoding human PBP maps to chromosome 4q12-q13.

Specifications

• Accession Number: NP
• Assay Range: 80-50000 pg/mL
• Assay Sensitivity: 80 pg/mL
• Calibration Range: 80 pg/mL to 50000 pg/mL
• Content And Storage: Pre-coated 96-well plate, standard, detector antibody, streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers. May be stored at 2–8°C for up to 6 months from the date of shipment.
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
• Sufficient For: 96 assays
• Target Species: Rat
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 2400003M24Rik; AI854500; Beta-TG; Beta-thromboglobulin; b-TG1; chemokine (C-X-C motif) ligand 7; connective tissue-activating peptide III; Connective tissue-activating peptide III(1-81); CTAP3; CTAPIII; CTAP-III; CTAP-III(1-81); CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7; LA-PF4; LDGF; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGF; NAP-2; NAP-2(1-63); NAP-2(1-66); NAP-2(73); NAP-2(74); NAP-2-L1; neutrophil activating peptide-2; neutrophil-activating peptide 2; Neutrophil-activating peptide 2(1-63); Neutrophil-activating peptide 2(1-66); Neutrophil-activating peptide 2(73); Neutrophil-activating peptide 2(74); neutrophil-activating peptide-2; PBP; Platelet basic protein; PPBP; pro-platelet basic protein; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); SCYB7; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; small-inducible cytokine B7; TC1; TC-1; TC2; TC-2; TGB; TGB1; THBGB; THBGB1; thrombocidin 1; thrombocidin 2; thromboglobulin, beta-12400003M24Rik; AI854500; Beta-TG; Beta-thromboglobulin; b-TG1; chemokine (C-X-C motif) ligand 7; connective tissue-activating peptide III; Connective tissue-activating peptide III(1-81); CTAP3; CTAPIII; CTAP-III; CTAP-III(1-81); CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7; LA-PF4; LDGF; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGF; NAP-2; NAP-2(1-63); NAP-2(1-66); NAP-2(73); NAP-2(74); NAP-2-L1; neutrophil activating peptide-2; neutrophil-activating peptide 2; Neutrophil-activating peptide 2(1-63); Neutrophil-activating peptide 2(1-66); Neutrophil-activating peptide 2(73); Neutrophil-activating peptide 2(74); neutrophil-activating peptide-2; PBP; Platelet basic protein; PPBP; pro-platelet basic protein; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); SCYB7; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; small-inducible cytokine B7; TC1; TC-1; TC2; TC-2; TGB; TGB1; THBGB; THBGB1; thrombocidin 1; thrombocidin 2; thromboglobulin, beta-1; Cxcl7; Nap-2
• Gene ID (Entrez): 246358
• Gene Symbol: PPBP
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Immunology
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: Cxcl7, Nap-2
• Target: PPBP
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min