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TE buffer, pH 8.0, RNAse free

Catalog No. AAJ62745AK
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125 mL
250 mL
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Catalog No. AAJ62745AK Supplier Thermo Scientific Chemicals Supplier No. J62745AK
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Description

CAS: 1185-53-1 | C4H12ClNO3 | 157.594 g/mol

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.

Solubility
Not miscible or difficult to mix in water.

Notes
200mM Tris-HCl with 20mM EDTA
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 1185-53-1
Molecular Formula C4H12ClNO3
Molecular Weight (g/mol) 157.594
MDL Number MFCD00236359
InChI Key QKNYBSVHEMOAJP-UHFFFAOYSA-N
PubChem CID 93573
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
SMILES C(C(CO)(CO)N)O.Cl

Specifications

pH 8.0
Physical Form Liquid
Quantity 250 mL
Solubility Information Not miscible or difficult to mix in water.
Chemical Name or Material TE buffer, RNAse free

Safety and Handling

TSCA Yes
Recommended Storage Ambient temperatures
SDS

RUO – Research Use Only

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