Invitrogen™ Human TEK (TIE2) [R849W], GST Tag, His Tag Recombinant Protein

Catalog No.	Quantity
PV6228	1 mg
PV6227	100 μg
PV6226	10 μg

Catalog No. PV6228 Supplier Invitrogen™ Supplier No. PV6228

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Human TEK (TIE2) R849W, C-terminal fragment, amino acids Q771-A1124 (as in NCBI/Protein entry NP_000450.1) with a R849W mutation, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM HEPES with 5mM DTT, 20% glycerol, 100mM NaCl, 15mM glutathione and no preservative; pH 7.5
• Molecular Weight (g/mol): 69 kDa
• Name: Human TEK (TIE2) [R849W], GST Tag, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: TEK, TIE-2
• Common Name: TIE2 (TEK) (R849W Mutant)
• Protein Length: aa 771-1124
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Sequence: 771-1124
• Content And Storage: Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Kinase Group: Receptor Tyrosine Kinase
• Expression System: Insect cells
• Protein Form: Recombinant
• Form: Liquid