Invitrogen™ Testosterone High Sensitivity Competitive ELISA Kit

Catalog No.	Quantity
EIATES	96 Tests

Catalog No. EIATES Supplier Invitrogen™ Supplier No. EIATES

Description

ELISA

The multispecies Testosterone ELISA with improved sensitivity quantitates testosterone in serum, plasma, dried fecal samples, or urine. Principle of the method: The Competitive ELISA research-use-only kit is designed to quantitatively measure testosterone independent of species. A testosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with donkey anti-sheep IgG antibodies. A monoclonal sheep antibodies and testosterone-peroxidase conjugate is added to the wells. The testosterone conjugate and any testosterone in the sample will compete to bind to the sheep testosterone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound testosteron-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of testosterone present in the sample. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.

Specifications

• Assay Range: 7.8-1000 pg/mL
• Assay Sensitivity: 2.97 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chromone
• Sample Type: Fecal Extract, Plasma, Serum, Urine
• Target Species: Chemical
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Androderm; C19H28O2; Mertestate; Sustanon; Testoderm; TestosteronAndroderm; C19H28O2; Mertestate; Sustanon; Testoderm; Testosteron;
• Interassay CV: <11%
• Intraassay CV: <6%
• Kit Contents: Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers, Dissociation Reagent, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Protein Aliases: 17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone, Testosterone
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Urine 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: -20°C
• Target: Testosterone
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min