Molecular Probes™ Tetramethylrhodamine (TAMRA) Alkyne (5-Carboxytetramethylrhodamine, Propargylamide), 5-isomer

Catalog No.	Quantity
T10183	0.5 mg

Catalog No. T10183 Supplier Molecular Probes™ Supplier No. T10183

Description

Tetramethylrhodamine (TAMRA) Alkyne (5-Carboxytetramethylrhodamine, Propargylamide), 5-isomer

The red-fluorescent tetramethylrhodamine (TAMRA) alkyne can be reacted with azides via a copper-catalyzed click reaction. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur – the label and detection tags react selectively and specifically with one another. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.

Specifications

• Format: Solid
• Quantity: 0.5 mg
• Product Type: Tetramethylrhodamine Alkyne
• Molecular Weight (g/mol): 467.53 Da
• Solubility: DMSO (Dimethylsulfoxide)
• Content And Storage: Store at ≤-20°C, desiccated and protected from light.
• Chemical Reactivity: Azide
• Detection Method: Fluorescence
• Reactive Group: Alkyne
• Shipping Condition: Room Temperature
• Reactive Moiety: Alkyne
• Label or Dye: TAMRA™ Isomers, TMR (Tetramethylrhodamine)
• Label Type: Classic Dyes
• Color: Orange
• Product Line: Molecular Probes

Frequently Asked Questions (FAQs)

I am using Click-iT AHA (L-azidohomoalanine) kit to label nascent proteins in live cells, then detecting with TAMRA alkyne after fixation and permeabilization and a click reaction. But I'm seeing nucleolar labeling in the cells. It this expected?

Yes. All proteins synthesized during the time the AHA is present will be detected, and they may be all over the cell. Our imaging shows strong labeling in the nucleoli and cytoplasm, as well as nuclear labeling.

For Research Use Only. Not for use in diagnostic procedures.