Thalidomide-4-OH (CAS No 5054-59-1) is a hydroxylated derivative of thalidomide studied primarily in the fields of pharmacology and oncology Originating from modifications of thalidomide this compound is designed to explore immunomodulatory and anti-inflammatory effects through various mechanisms such as inhibiting angiogenesis and modulating cytokine production It targets key pathways involving TNF-alpha and exhibits significant activity in the nanomolar to low micromolar range Thalidomide-4-OH is used in cell-based assays and animal studies to investigate therapeutic potentials and side effects often as part of drug screening programs Its application includes studying its efficacy in cancer cell models and exploring its potential benefits in autoimmune and chronic inflammatory diseases Experimental concentrations typically depend on the specific research design