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Bachem DABCYL-g-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS
Supplier: Bachem 4030716.0001
DABCYL-g-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS Trifluoroacetate, 127134-13-8, C73H97N17O18S, Mr 1532.74, 1mg. The fluorogenic HIV-1 protease substrate DABCYL-Gaba-SQNYPIVQ-EDANS consists of an octapeptide with a fluorescent donor (EDANS) and a quenching acceptor (DABCYL), attached at the COOH- and NH2-termini. The g-Abu spacer was inserted to avoid potential steric hindrance of substrate binding by the bulky acceptor. This FRET substrate is cleaved by the HIV-1 protease at the Tyr-Pro bond which results in a time-dependent increase in fluorescence intensity; excitation at 340 nm, emission at 490 nm.
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