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Thermo Scientific™ EZ-Link™ NHS-PEG Solid-Phase Biotinylation Kit - Mini-Spin Columns

A column-based approach to antibody biotinylation and clean-up

Manufacturer:  Thermo Scientific™ 21450

Catalog No. PI21450

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Includes: Kit with Prepacked format (1 to 10mg IgG): Immobilized Nickel Chelate Column 1mL, BupH Phosphate Buffered Saline 1 pack (makes 500mL), No-Weigh NHS-PEG4-Biotin Microtubes 8 x 2mg, 4M Imidazole Stock Solution 5mL
Kit with Mini-Spin format (0.1 to 1mg IgG): Nickel Chelated SwellGel Discs (pack of 10), Mini-Spin columns (pack of 10), 2mL Microcentrifuge tubes (pack of 30), BupH Phosphate Buffered Saline 1 pack (makes 500mL), No-Weigh NHS-PEG4-Biotin Microtubes 8 x 2mg, 4M Imidazole Stock Solution 5mL



This innovative antibody-labeling system uses nickel-chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions. Once held in place on the resin, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and byproducts are then washed away before recovering the labeled and purified antibody from the resin using a mild imidazole solution. No gel filtration or dialysis is needed. Four kits are available for small (0.1-1mg) or large (1-10mg) antibody samples using either amine-directed (NHS ester) or sulfhydryl-directed (maleimide) labeling reagents.


  • Fast labeling and purification - the entire procedure takes only one hour (two hours for sulfhydryl labeling kits)
  • Easy removal of spent and excess labeling reagent - simply wash away the reaction byproducts - no need for dialysis or gel filtration
  • No dilution effects - solid-phase method allows initially dilute antibodies to be recovered in a smaller volume after labeling
  • Optimized protocols - specific protocols for antibody ensure appropriate level of labeling (2-5 biotins per antibody molecule), minimizing possibility of inactivation caused by overlabeling
  • Sufficient reagents for eight biotin-labeling experiments - Thermo Scientific No-Weigh Packaging ensures that the biotin reagent is fully active for eight separate experiments



Antibodies (IgG)
0.5-1 mg
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Safety and Handling

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