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Thermo Scientific™ MagJET NGS Cleanup and Size Selection Kit
Description
Thermo Scientific MagJET NGS Cleanup and Size Selection kit is designed for efficient and robust DNA fragment library cleanup and size-selection from a variety of enzymatic reaction mixtures including PCR, ligation, adapter addition and DNA end-repair reaction mixes. The kit utilizes high-capacity MagJET paramagnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. The purified DNA fragments are free of any next-generation sequencing workflow inhibiting components, such as sequencing adapters, primer dimers, unincorporated nucleotides, enzymes or salts, and are ready-to-use in downstream NGS applications. No prior DNA fragment library purification is required before the cleanup and size-selection procedures.
The kit is suitable for cleanup or size selection of 5 ng to 5 μg of DNA fragment library. MagJET cleanup and adapter removal protocols can easily be automated for higher throughput on automatic platforms such as Thermo Scientific KingFisher Flex and Thermo Scientific KingFisher Duo.
Highlights
• Robust—reliable cleanup and size selection from different reaction mixtures and buffers
• Flexible—user-defined size-selection of exact DNA fragment range
• NGS-grade purity—complete removal of NGS adapters, primers, nucleotides, enzymes and other reaction components inhibiting NGS workflow
• High throughput compatible—easy to automate on the Thermo Scientific KingFisher magnetic particle processors for high-throughput sample handling
Applications
DNA fragment library cleanup and size-selection for downstream use in next generation sequencing workflows.
Includes
• MagJET Magnetic Beads
• Binding Solution
• Wash Buffer (concentrated)
• Elution Buffer
• DNA Fragment Mix
Specifications
Specifications
| Content And Storage | • 1 mL DNA Fragment Mix; -20°C • 110 μL MagJET Magnetic Beads; 4°C • 8 mL Binding Buffer (concentrated); room temperature • 3 mL Wash Buffer (concentrated); room temperature • 2 x 1.5 mL Elution Buffer; room temperature |
| Isolation Technology | Magnetic Bead |
| Sample Type | Enzymatic Reactions (PCR, ligation, adapter addition, DNA end-repair reaction) |
| Elution Volume | 10 to 50 μL |
| Final Product Type | DNA |
| For Use With (Equipment) | KingFisher™ Flex, KingFisher™ Duo Prime |
| For Use With (Application) | PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization |
| High-throughput Compatibility | High-throughput Compatible |
| Quantity | 10 Preps |
| Shipping Condition | Room Temperature |
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Frequently Asked Questions (FAQs)
We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.
No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.
No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.
Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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