Thermo Scientific™ GeneJET™ Plasmid Maxiprep Kit 

The GeneJET Plasmid Maxiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures.

Manufacturer:  Thermo Scientific™ K0861

Catalog No. FERK0861

Includes: Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET Maxi Purification Columns pre-assembled with collection tubes (50mL) and Collection tubes (50mL).

Description

GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750µg of high-copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

• Efficient - high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
• High concentration of DNA purified - > 400 ng/µL
  ·Convenient - spin column format, centrifuge or vacuum protocols
• Fast - procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription

Includes

• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Endotoxin Binding Reagent
• Wash Solution I (concentrated)
• Wash Solution II (concentrated)
• RNase A Solution
• Elution Buffer (10 mM Tris-HCl, pH 8.5)
• GeneJET Maxi Purification Columns pre-assembled with collection tubes (50 mL)
• Collection tubes (50 mL)

Hazardous: No
Quality Control:

• The GeneJET Plasmid Maxiprep Kit is qualified by isolating high copy number plasmid DNA from 250 mL of overnight E. coli culture (OD600 = 2) grown in LB medium following the protocol outlined in the manual.
• The kit passes QC requirements if the purified plasmid DNA has an A_260/280 ratio between 1.8 and 1.9 and a dominant band of supercoiled plasmid DNA is observed after agarose gel electrophoresis.
• The functional quality of the plasmid DNA is evaluated by digestion with restriction endonucleases.

Storage Condition: Ambient

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription

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Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

• Description: Plasmid Maxiprep Kit; 10 preps
• Quantity: 10 preps
• Storage Requirements: Store at room temperature