Thermo Scientific™ GeneJET Whole Blood RNA Purification Mini Kit

Description

Includes

Lysis Buffer, Wash Buffer WB 1 (concentrated), Wash Buffer 2 (concentrated), Water, nuclease-free, GeneJET™ RNA Purification Columns (pre-assembled with collection tubes), Collection tubes (2mL), Collection tubes (1.5mL), Detailed Protocol

Designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids

Thermo Scientific GeneJET Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.

Highlights

• Efficient – up to 1.8 μg of total RNA from 500 μL of human blood
• Convenient – spin columns are capped and assembled with collection tubes
• Pure – RNA pure from enzyme inhibitors, proteins, ready to use in all demanding applications. Validated in cDNA synthesis and quantitative RT-PCR
• Fast and simple – procedure takes less than 15 minutes and does not involve organic extraction or differential RBC lysis
• Compatible – suitable for vide variety of samples - human and animal blood treated with EDTA, citrate or heparin, buffy coat, bone marrow, and related body fluids

Applications

• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assaysncludes Lysis Buffer Wash Buffer WB 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with collection tubes) Collection tubes, 2 mL Collection tubes, 1.5 mL Detailed Protocol

Specifications

• Content And Storage: • 40 mL Lysis Buffer; store at room temperature
  • 40 mL Wash Buffer WB1 (concentrated); room temperature
  • 23 mL Wash Buffer 2 (concentrated); room temperature
  • 30 mL Nuclease-free Water; room temperature
  • 50 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
  • 50 Collection Tubes (2 mL); room temperature
  • 50 Collection Tubes (1.5 mL); room temperature
• Isolation Technology: Silica Spin Column
• Purification Time: 15 min. (After sample homogenization)
• Elution Volume: 50 μL
• Sample Type: Blood, Bone Marrow, Buffy Coat
• Final Product Type: Total RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Quantity: 50 Preps
• Scale: Mini
• Shipping Condition: Room Temperature
• Starting Material Amount: Blood: 50–500 μL, Bone Marrow: 50–500 μL, Buffy Coat: 500 μL
• Yield: Up to 11 μg

For Research Use Only. Not for use in diagnostic procedures.