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Thioglycollate Broth (THIO) is an enriched nonselective liquid medium used for the growth of microaerophilic and anaerobic bacteria, including fastidious organisms, from clinical specimens. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.THIO is an enriched nonselective liquid medium used for the growth of microaerophilic and fastidious anaerobic organisms. THIO contain casein, meat peptones, yeast extract, and dextrose as the nutritive base. Sodium thioglycollate and L-cystine are included as reducing agents. Hemin and vitamin K1 are included to promote the growth of fastidious anaerobes. A small quantity of agar is added to slow the diffusion of oxygen. A marble chip is included to each tube to act as a pH buffer and neutralize acids produced during growth of the organisms.