Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Miscellaneous Immobilization Supports
  6. Thiopropyl Resin, G-Biosciences 

Thiopropyl Resin, G-Biosciences
SCI_ED

Catalog No. S001122
Change view
Click to view available options
Quantity:
5 mL
25 mL
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
S001122 25 mL
S001121 5 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. S001122 Supplier G-Biosciences Supplier No. 7862224
Only null left
Add to Cart
Add to Cart

Description

Thiopropyl Resin is used in purification of thiol group containing proteins, molecules and enzymes.

The thiol-containing molecules are reversibly immobilized on the resin. When the sample to be purified is passed through the resin, thiol-containing biomolecules bind to the resin to form mixed disulfides and release 2-thiopyridone. The bound molecule is eluted by addition of reducing agent such as DTT or 2-mercaptoethanol.

Specifications:

  • Active group: 2-pyridyl disulfide
  • Active group concentration: 18-31 μmole 2-pyridyl disulfide/ml drained gel
  • Spacer: 3-Carbon-SH group
  • Coupling capacity: around 10-20 mg protein/mL of drained gel
  • Matrix: 6% cross-linked Agarose
  • pH stability: 2-8 long term, 2-8 for CIP
  • Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Do not use azides.
  • Physical stability: volume variations are negligible for changes in pH or ionic strength
  • Supplied as lyophilized powder. To rehydrate, add twice the final resin volume of binding/storage buffer and allow to swell for 15 min.

Specifications

Particle Size 45 to 165 μm
Form Dry
Product Line Thermo Scientific
Quantity 25 mL
Type Methacrylate (ECR Resin)
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.