ACROBiosystems ACROBiosystems Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin
Manufacturer: ACROBiosystems TITH5253100UG
Recombinant Protein;100UG;Human TIGIT, Mouse IgG2a Fc Tag, low endotoxin (TIT-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # AAI01290).;This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.1 kDa. The protein migrates as 42-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human CD155, Fc Tag (Cat. No. CD5-H5251) at 5 μg/mL (100 μL/well) can bind Human TIGIT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TIT-H5253) with a linear range of 0.5-8 ng/mL (QC tested).;Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, Mouse IgG2a Fc Tag (Cat. No. TIT-H5253): Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) binding reactions. The assay was performed according to the above described protocol.
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