Invitrogen™ Rat TIMP-1 ELISA Kit

ELISA

Supplier:  Invitrogen™ ERTIMP1

Catalog No. 50-254-5321

Description

The Rat Tissue Inhibitor of Matrix MetalloProteinase 1 (Rt TIMP-1) ELISA quantitates Rt TIMP-1 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt TIMP-1. Principle of the method The Rat TIMP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.

Specifications

• Accession Number: P30120
• Assay Sensitivity: 4 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 116510
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Oncology
• Storage Requirements: 2°C to 8°C
• Target: TIMP1
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 4.1 to 3000 pg/mL
• Calibration Range: 4 pg/mL to 3000 pg/mL
• Conjugate: HRP
• Protein Family: Cytokines & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, LIX, Leptin, MCP-1, MIP-3 alpha, beta- NGF, TNF-alpha, VEGF.
• Target Species: Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Timp1
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma 3 μL, Serum 3 μL, Supernatant 100 μL
• Synonym: TIMP-1, Timp
• Test Time: 1 hrs 20 mins