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Thermo Scientific™ Tissue Protein Extraction Bundle

Catalog No. PIB90002
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Catalog No. PIB90002 Supplier Thermo Scientific™ Supplier No. B90002

Description

Includes

T-PER Tissue Protein Extraction Reagent (500 mL), Pierce Protease Inhibitor Mini Tablets (30 tablets), Pierce Rapid Gold BCA Protein Assay Kit (250 mL)

Tissue Protein Extraction Bundle enables mild extraction of total protein from tissue samples and provides high-precision total protein concentration determination. The bundle includes T-PER Tissue Protein Extraction Reagent, Pierce Protease Inhibitor Mini Tablets and Rapid Gold BCA Protein Assay

  • T-PER tissue cell lysis reagent utilizes a mild detergent in 25mM bicine, 150mM sodium chloride (pH 7.6) to maximize the efficiency of protein solubilization from mammalian tissue samples by homogenization. Cell lysates prepared with T-PER Reagent are directly compatible with reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures
  • Pierce Protease Inhibitor Mini Tablets protects proteins from proteolytic degradation through inhibition of serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples
  • Pierce Rapid Gold BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay optimized to measure (at 480 nm) total protein concentration compared to a protein standard curve of known concentrations. The assay uses the same copper-chelating technology as the well-known traditional Pierce BCA Protein Assay and provides comparable accuracy but with a 5 minute, room temperature incubation. This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results
TRUSTED_SUSTAINABILITY

Specifications

Quantity 1 Bundle
Includes T-PER Tissue Protein Extraction Reagent (500 mL), Pierce Protease Inhibitor Mini Tablets (30 tablets), Pierce Rapid Gold BCA Protein Assay Kit (250 mL)
Content And Storage See instructions for individual components
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