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AdipoGen TMB dihydrochloride
Supplier: AdipoGen CDXT0151G001
Chemical. CAS 64285-73-0. Formula C16H20N2 . 2HCl. MW 240.3 . 72.9. Synthetic TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green lambdamax 655 nm in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays ELISA. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 resulting in a yellow color, and read at 450nm.
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