ProSci TNF-alpha Recombinant Protein

Catalog No.	Quantity
16-838-532	0.01 mg

Catalog No. 16-838-532 Supplier ProSci Supplier No. 401150010

Description

Recombinant Protein

TNF-α is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Specifications

• Accession Number: NP_000585.2
• Gene ID (Entrez): 7124
• Quantity: 0.01 mg
• Source: E. coli
• Common Name: tumor necrosis factor
• Gene Symbol: TNF
• Biological Activity: The ED50 was determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
• Content And Storage: The lyophilized TNF-alpha recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted TNF-alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing TNF-alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein (at a cool and stable temperature.)
• Purity or Quality Grade: >98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng / μg (1 EU/μg).
• Synonym: DIF, TNFA, TNFSF2, TNF-alpha, Tumor necrosis factor, Cachectin, TNF-a