Invitrogen™ Rhesus/Cynomolgus Monkey TNF-alpha Recombinant Protein

Catalog No.	Quantity
A42630	5 μg
A42631	100 μg

Catalog No. A42630 Supplier Invitrogen™ Supplier No. A42630

Description

Recombinant Protein

Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Apportion the reconstituted protein into working aliquots and store at -20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA. Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycle.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Accession Number: P48094, P79337
• For Use With (Application): Control, Bioactivity, Immunohistochemistry, Western Blot
• Formulation: 50mM tris with 50mM NaCl, 5% trehalose, 5% mannitol, 0.01% Tween 80 and no preservative; pH 8
• Gene ID (Entrez): 102139631, 715467
• Molecular Weight (g/mol): 17.4 kDa
• Name: Rhesus/Cynomolgus Monkey TNF-alpha
• pH Range: 8
• Purification Method: Purified
• Quantity: 5 μg
• Source: E. coli
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: RUO
• Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
• Common Name: TNF alpha
• Gene Symbol: Tnf
• Biological Activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 2-20 pg/mL
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Monkey
• Recombinant: Recombinant
• Protein Tag: Untagged
• Sequence: Cynomologus/rhesus TNF, amino acids Val77-Leu233 (Accession # NP_001040614.1) with an N-terminal Met (Cynomolgus and rhesus TNF sequences are identical)
• Content And Storage: Includes 1 vial of lyophilized protein. Store at -20 °C.
• Activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 2-20 pg/mL.
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Form: Lyophilized
• Protein Subtype: TNF (Tumor Necrosis Factor)
• Research Category: Apoptosis, Cell Cycle & Proliferation, Inflammation, Oncology
• Purity or Quality Grade: >90% by SDS-PAGE
• Protein: tumor necrosis factor