Invitrogen™ Mouse TNFR1 (Soluble) ELISA Kit

Catalog No.	Quantity
EMTNFRSF1A	96 Tests
50-255-0922	10 x 96 Tests

Catalog No. EMTNFRSF1A Supplier Invitrogen™ Supplier No. EMTNFRSF1A

Description

ELISA

The Mouse soluble TNFRI (TNFRSF1A) ELISA quantitates Ms TNFRI in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms sTNFRI. Principle of the method The Mouse TNF-RI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.

Specifications

• Accession Number: P25118
• Assay Range: 1.23 to 300 pg/mL
• Assay Sensitivity: 1 pg/mL
• Calibration Range: 1 pg/mL to 300 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Cytokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects mouse sTNFRI. Other species not determined.
• Sufficient For: 96 Assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 21937
• Gene Symbol: Tnfrsf1a
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Plasma 20 μL, Serum 20 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: CD120a, FPF, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNF-alphaR1, TNFAR, TNFR60, TNFRI, TNFRp55, TNFalpha-R1, Tnfr-2, Tnfr1, p55, p55-R
• Target: TNFR1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins