Invitrogen™ Human TNFRII (Soluble) ELISA Kit

Catalog No.	Quantity
KAC1771	96 Tests

Catalog No. KAC1771 Supplier Invitrogen™ Supplier No. KAC1771

Description

ELISA

The Human Tumor Necrosis Factor Receptor II (Hu TNFRII) ELISA quantitates Hu TNFR1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFRII. Principle of the method The Human TNFRII solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.

Specifications

• Accession Number: P20333
• Assay Range: 2-142 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody(s), diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cytokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CD120b; Etanercept; p75; p75 TNF receptor; p75 TNFR; p75TNFR; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR1B variant 1; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TBP-2; TBPII; TNF receptor beta chain; TNF receptor superfamily member 1B; TNF-alphaR2; TNFalpha-R2; TNFBR; TNFR; TNFR II; Tnfr-1; TNFR1B; TNFR2; TNF-R2; Tnfr-2; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor; tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor superfamily member 1b, membrane form; tumor necrosis factor receptor superfamily, member 1B; tumor necrosis factor receptor type II; Tumor necrosis factor-binding protein 2CD120b; Etanercept; p75; p75 TNF receptor; p75 TNFR; p75TNFR; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR1B variant 1; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TBP-2; TBPII; TNF receptor beta chain; TNF receptor superfamily member 1B; TNF-alphaR2; TNFalpha-R2; TNFBR; TNFR; TNFR II; Tnfr-1; TNFR1B; TNFR2; TNF-R2; Tnfr-2; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor; tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor superfamily member 1b, membrane form; tumor necrosis factor receptor superfamily, member 1B; tumor necrosis factor receptor type II; Tumor necrosis factor-binding protein 2; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; p75TNFR
• Gene ID (Entrez): 7133
• Gene Symbol: Tnfrsf1b
• Incubation Time: 1.25 hr.
• Interassay CV: 9.2%
• Intraassay CV: 2.9%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
• Label or Dye: HRP
• Marker Gene: TNF-RII
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: TNFR2
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 15 min