Invitrogen™ Human TNFRSF18 (GITR) ELISA Kit

Catalog No.	Quantity
50-274-1194	10 x 96 Tests

Catalog No. 50-274-1194 Supplier Invitrogen™ Supplier No. EHTNFRSF18X10

Description

ELISA

The Human TNFRSF18 (GITR) ELISA quantitates Hu GITR in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GITR. Principle of the method The Human GITR solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Specifications

• Accession Number: Q9Y5U5
• Assay Range: 15-10000 pg/mL
• Assay Sensitivity: 15 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Activation-inducible TNFR family receptor; AITR; CD357; GITR; GITR-D; glucocorticoid-induced TNFR-related protein; PRO364; TNF receptor superfamily activation-inducible protein; TNF receptor superfamily member 18; TNFRSF18; Tumor necrosis factor receptor superfamily member 18; tumor necrosis factor receptor superfamily, member 18; UNQ319; UNQ319/PRO364Activation-inducible TNFR family receptor; AITR; CD357; GITR; GITR-D; glucocorticoid-induced TNFR-related protein; PRO364; TNF receptor superfamily activation-inducible protein; TNF receptor superfamily member 18; TNFRSF18; Tumor necrosis factor receptor superfamily member 18; tumor necrosis factor receptor superfamily, member 18; UNQ319; UNQ319/PRO364; AITR; CD357; GITR; GITR-D
• Gene ID (Entrez): 8784
• Gene Symbol: Tnfrsf18
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: GITR
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min