Invitrogen™ Mouse TNFSF4 (OX40L) ELISA Kit

Description

ELISA

The Mouse TNFSF4 (OX40L) ELISA quantitates Ms TNFSF4 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TNFSF4. Principle of the method The Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

Specifications

• Accession Number: P43488
• Assay Range: 32.77 to 8000 pg/mL
• Assay Sensitivity: 30 pg/mL
• Calibration Range: 30 pg/mL to 8000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Cytokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects mouse TNFSF4/OX40 Ligand and on cross reactivity with rmBAFF, rmEDA, rmGITR Ligand, rmFas Ligand, rmLIGHT, rmTWEAK, rhAPRIL, or rhTNFSF4.
• Sufficient For: 96 assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 22164
• Gene Symbol: Ath-1,Ath1,CD134L,OX-40 L,O x 40 L,TXGP1,Tnlg2b,Txgp1l,gp34
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Ath-1, Ath1, CD134L, OX-40 L, O x 40 L, TXGP1, Txgp1l, gp34
• Target: OX40L (CD252)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins