Invitrogen™ Human TNK2 (ACK), GST Tag Recombinant Protein

Catalog No.	Quantity
PV4807	10 μg

Catalog No. PV4807 Supplier Invitrogen™ Supplier No. PV4807

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Recombinant, Catalytic Domain.

TNK2 (ACK) (activated Cdc42-associated kinase) is a non-receptor tyrosine kinase that signals downstream of Cdc42. TNK2 is known to be involved in tumorigenesis and metastasis, particularly in prostate cancer. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.

Specifications

• Accession Number: Q07912
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 2mM DTT, 150mM NaCl, 0.02% Triton X-100, 50% glycerol, 0.5mM EDTA and no preservative; pH 7.5
• Gene ID (Entrez): 10188
• Molecular Weight (g/mol): 69.3 kDa
• Name: Human TNK2 (ACK), GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: ACK; ACK1; Ack-1; Activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; Cdc42 GTPase-inhibiting protein; Cdgip; Non-receptor protein tyrosine kinase Ack; p21cdc42Hs; P21cdc42Hs kinase; Pyk1; Tnk2; tnk2 {ECO:0000250; tyrosine kinase non receptor 2; tyrosine kinase non-receptor protein 2; tyrosine kinase, non-receptor, 2; UniProtKB:Q07912}
• Common Name: ACK1
• Gene Symbol: TNK2
• Kinase Family: ACK Kinase Family
• Protein Length: aa 110-476
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 110-476
• Content And Storage: Storage Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, 0.02% Trition X-100, 0.5 mM EDTA, 2 mM DTT, 50% Glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Assay Entry: Biochemical binding/kinetic, Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity
• Kinase Group: Tyrosine Kinases
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate
• Target Entry: TNK2
• Target Gene: tyrosine kinase, non-receptor, 2
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Cytoplasmic, Catalytic
• Form: Liquid
• Protein Subtype: Cytoplasmic Tyrosine Kinases
• Research Category: Oncology, Signal Transduction
• Purity or Quality Grade: 95% by SDS-PAGE