Abnova™ TOP2A/CEN17 CISH Probe

Description

TOP2A/CEN17 CISH Probe is designed for the qualitative detection of human TOP2A gene and chromosome 17 alpha satellites in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green colored distinct dots (TOP2A gene region), and dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (CEN 17).
Normal situation: In interphases of normal cells or cells without an amplification involving the TOP2A gene locus, two green signals and two red signals appear.
Aberrant situation: In cells with amplification of the TOP2A gene locus an increased number of gene specific signals or signal clusters are visible. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P