LUNA NANOTECH INC Total Rna Purification Kit, 250 Preps

Supplier:  LUNA NANOTECH INC NK051250

PuroSPIN™ Total RNA Purification Kit, 250 Preps

PuroSPIN™ Total RNA Purification Kitis designed to purify total RNA from bacteria, mammalian cells, yeast, or animal tissue. It greatly simplifies the extraction process by incorporating a convenient easy-to-use extraction technology based on nucleic acid binding silica spin-columns. The RNA is first released form the cells by the treatment with the Lysis Buffer LB-R. For RNA extraction from bacterial cell the lysis step is preceded by the treatment with the Lysozyme enzyme to disrupt bacterial cell wall. RNA is then loaded onto silica-based spin columns and washed to remove protein, salt and other contaminants. The kit includes on-column DNase I digestion step to remove any contaminating DNA. Finally, purified RNA is eluted from the column into a clean microcentrifuge tube in Elution Buffer EB, or nuclease-free water.

The kit can also be used to perform RNA cleanup after DNase I or other enzymatic treatm

Catalog No. 50-256-6960