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Innovative Research Inc Human Total Thrombin Antithrombin Complex ELISA Kit
Supplier: Innovative Research Inc IHUTATCXKTT
Human thrombin/antithrombin (TAT) complex antigen assay is intended for the quantitative determination of the covalent complex of thrombin and its inhibitor antithrombin III in human plasma and serum. For research use only. . Strip well format. Reagents for up to 96 tests.
Human TAT complex in samples will bind to the anti-human thrombin capture antibody coated on the microtiter plate. After appropriate washing steps, monoclonal anti-human antithrombin primary antibody binds to TAT complex captured on the plate. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of TAT complex. Color development is proportional to the concentration of TAT complex in the samples. Free thrombin, prothrombin and antithrombin III will not be detected by th
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