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TR-FRET Dilution Buffer

Catalog No. PV3574
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PV3574 100 mL
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Catalog No. PV3574 Supplier LIFE TECHNOLOGIES Supplier No. PV3574
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Description

TR-FRET Dilution Buffer

TR-FRET Dilution Buffer is an essential component for kinase assays using LanthaScreen™ technology. The TR-FRET Dilution Buffer is used to dilute LanthaScreen™ antibodies for screening applications.
TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material Dilution Buffer
Physical Form Liquid
Quantity 100 mL

Frequently Asked Questions (FAQs)

I have no assay window in my TR-FRET assay. What is the reason?

The single most common reason that a TR-FRET assay fails is that an incorrect choice of emission filters was made. Unlike other fluorescent assays, the filters used in a TR-FRET assay must be exactly those that are recommended for your instrument as the emission filter choice can make or break the assay. The excitation filter has more of an impact on the assay window. Please refer to our instrument setup guides in our instrument compatibility portal (https://www.thermofisher.com/instrumentsetup). If your instrument is not listed there, please contact Drug Discovery Technical Support at drugdiscoverytech@thermofisher.com.

Note: Test your microplate reader's TR-FRET setup before you begin any work with your assay. Using reagents that you have already purchased for your assay, you can test the microplate reader's TR-FRET setup. Please refer to the Terbium (Tb) Assay (http://tools.thermofisher.com/content/sfs/manuals/LanthaScreen_Tb_Instrument_Control_Application_Note17Sep10.pdf) and Europium (Eu) Assay (http://tools.thermofisher.com/content/sfs/manuals/Eu_Instrument_Control_AppNote_12Nov10.pdf) Application Notes for plate reader setup.

For Research Use Only. Not for use in diagnostic procedures.

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