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Sigma Aldrich Fine Chemicals Biosciences TranscreenerR ADP2 fP Assa
Supplier: Sigma Aldrich Fine Chemicals Biosciences 30101K
The Transcreener(R) HTS Assay platform overcomes the need for time-consuming one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener(R) HTS Assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener(R) ADP2 fP Assay is a far-red competitive fluorescence polarization (fP) assay based on the detection of ADP and therefore is compatible with any enzyme class that produces ADP including protein lipid and carbohydrate kinases ATPases DNA helicases carboxylases and glutamine synthetase. The Transcreener(R) ADP2 Assay is a simple one step homogenous detection assay and is extremely flexible with regard to ATP concentration (0.1 to 1 000 muM ATP). The assay provides excellent signal at low substrate conversion
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