Invitrogen™ Human Trappin-2/PI3 ELISA Kit

Catalog No.	Quantity
EHPI3X5	5 x 96 Tests
EHPI3	96 Tests

Catalog No. EHPI3X5 Supplier Invitrogen™ Supplier No. EHPI3X5

Description

ELISA

The Human Trappin-2/PI3 ELISA quantitates Hu PI3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PI3. Principle of the method The Human PI3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Peptidase inhibitor 3 (PI3), also known as Elafin, is a gene located on chromosome 20q13.12, encoding a protein that functions as a serine protease inhibitor. Elafin is primarily expressed in epithelial tissues, including skin and mucosal surfaces, where it plays a protective role against excessive proteolytic activity by inhibiting enzymes like neutrophil elastase and proteinase 3. This activity is crucial for maintaining tissue homeostasis and preventing inflammatory damage. PI3 is regulated by pro-inflammatory cytokines and is involved in modulating inflammatory responses and tissue repair processes. Its expression is often elevated in conditions characterized by inflammation, such as chronic obstructive pulmonary disease (COPD), psoriasis, and other inflammatory diseases, reflecting its role in guarding against protease-induced tissue damage. Research into PI3 focuses on its potential therapeutic applications in inflammatory diseases and its utility as a biomarker for assessing inflammation-related pathologies.

Specifications

• Accession Number: P19957
• Assay Range: 5-5000 pg/mL
• Assay Sensitivity: 5 pg/mL
• Calibration Range: 5 pg/mL to 5000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Proteases Peptidases & Inhibitors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
• Sufficient For: 5 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: cementoin; Elafin; elastase-specific inhibitor; ESI; peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); protease inhibitor WAP3; SKALP; skin-derived antileukoproteinase; trappin-2; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14; WAP3; WFDC14cementoin; Elafin; elastase-specific inhibitor; ESI; peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); protease inhibitor WAP3; SKALP; skin-derived antileukoproteinase; trappin-2; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14; WAP3; WFDC14;
• Gene ID (Entrez): 5266
• Gene Symbol: PI3
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Other Research
• Sample Volume: Plasma 3.3 μL, Serum 3.3 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: ESI, SKALP, WAP3, WFDC14, cementoin
• Target: PI3
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min