U-Blot Pan-Tri-Methyllysine Rabbit pAb (KLH-conjugate immunogen) is a Rabbit-derived polyclonal antibody that specifically recognizes Tri-Methyllysine a lysine trimethylation modification at Lys(K) It shows reactivity with Species Independent Validated applications include ELISA WB IP IF IAC Recommended dilutions ELISA 12000 WB 12000 IP 5 ug/mg protein sample IF 5 ug/mL or 1100 Purified by n-(epsilon) trimethyllysine on agarose and immunoaffinity absorbed with n-(epsilon) methylated lysine and n-(epsilon) dimethylated lysine on agarose Histones undergo a wide range of enzyme-catalyzed post-translational modifications including acetylation methylation phosphorylation and ubiquitination Lysine trimethylation is a vital epigenetic modification that predominantly occurs on histone lysine residues It is dynamically regulated by histone methyltransferases and demethylases and extensively distributed in eukaryotic chromatin Supplied as 100 uL