Thermo Scientific Chemicals Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3

Description

CAS: 610769-35-2 | C14H30BN3O14 | 475.211 g/mol

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Chelation/Complexation reagent, pH buffer

Specifications

Chemical Identifiers

• CAS: 610769-35-2
• Molecular Formula: C14H30BN3O14
• Molecular Weight (g/mol): 475.211
• MDL Number: MFCD00236358
• InChI Key: OSBLTNPMIGYQGY-UHFFFAOYSA-N
• Synonym: 2-Amino-2-(hydroxymethyl)-1, 3-propanediol, TRIS, Tris buffer, Tromethamine, Tromethane
• PubChem CID: 21257725
• IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid
• SMILES: B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O

• Color: Colorless
• pH: 8.2 to 8.4 (25°C)
• Physical Form: Liquid
• Quantity: 1 L
• Packaging: Plastic bottle
• Beilstein: 04, 303
• Merck Index: 14, 9772
• Grade: Molecular Biology
• Chemical Name or Material: Tris Borate EDTA buffer, pH 8.3

Frequently Asked Questions (FAQs)

My Thermo Scientific brand TBE buffer has precipitated out of solution. What can I do?

Concentrations of 5X Tris Borate EDTA (TBE) buffer or greater may precipitate due to the nature of the product. This precipitation is a result of nucleation of salt crystals by dust particles or other insoluble materials. In some cases, we recommend heating the buffer to ~ 50°C to re-dissolve the TBE into solution. Alternately, to avoid precipitation of high concentration TBE buffers, after the solution is prepared, you can filter it through a 0.2 µm cellulose acetate or cellulose nitrate filter. These treatments have no deleterious effect on the buffering properties of TBE.

Safety and Handling

Hazard Category	Warning
Hazard Statement	GHS P Statement Causes skin irritation. Causes serious eye irritation.
Precautionary Statement	GHS P Statement Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. WARNING: The information provided on this web site was developed in compliance with European Union (EU) regulations and is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guide for safe handling and use. It is not to be considered as either a warranty or quality specification.

SDS

RUO – Research Use Only