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Description
- TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis.
- TBE is used with non-denaturing or denaturing (7 M urea) gels.
- It is also routinely used for DNA automated sequencing gel.
- TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
- It is used to prepare a 1 X buffer for polyacrylamide and agarose gel electrophoresis.
- TBE buffer is a solution of 1M Tris, 0.9M boric acid and 0.01M EDTA disodium salt, prepared from MP's Ultra Pure products.
Specifications
Specifications
| pH | 8.3 to 8.5 (as is); 8.23 to 8.43 (1X) |
| Physical Form | Solution |
| Quantity | 1 L |
Safety and Handling
| storageNote1 | Store at Room Temperature(15-30°C). |
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