Tris-HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).
Use the Tris-HCl 0.5 M (pH 6.8) buffer for:
— stacking gels
— resolving gels
— DEAE Dextran-mediated transfection of non-adherent and adherent cells
Applications: Tris-HCl (2M), pH 6.8 is useful with SDS-PAGE and Electrophoresis.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
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