Invitrogen™ Human Troponin T (TNNT1) ELISA Kit

Catalog No.	Quantity
EHTNNT1X5	5 x 96 Tests
EHTNNT1	96 Tests

Catalog No. EHTNNT1X5 Supplier Invitrogen™ Supplier No. EHTNNT1X5

Description

ELISA

The Human Troponin T (TNNT1) ELISA quantitates Hu TNNT1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNNT1. Principle of the method The Human TNNT1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: P13805
• Assay Range: 0.391-25 ng/mL
• Assay Sensitivity: 0.35 ng/mL
• Calibration Range: 0.35 ng/mL to 25 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM-9, Angiopoietin 2, APRIL, BMP-2, BMP-9, C5a, Cathepsin L, CD200, CD97, Chemerin, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGF R, IFN-gamma, IGF-2, IGF-2 R, IL-1R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV, Osteoactivin, PD-1, PGRP-5, Serpin A4, sFRP-3, Thrombomodulin, TLR2, TRAIL R1, WIF-1
• Sufficient For: 5 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ANM; AW146156; Fang2; MGC104241; NEM5; skeletal muscle slow-twitch TnT; slow skeletal muscle troponin T; ssTnT; sTnT; Tnnt1; TNT; TNTS; troponin T slow skeletal muscle type; troponin T slow type isoform sTnT1; troponin T slow type isoform sTnT2; troponin T type 1 (skeletal, slow); troponin T, slow skeletal muscle; troponin T1, skeletal, slow; troponin T1, slow skeletal type; troponin-T1, skeletal, slow; zgc:193831; zgc:193865ANM; AW146156; Fang2; MGC104241; NEM5; skeletal muscle slow-twitch TnT; slow skeletal muscle troponin T; ssTnT; sTnT; Tnnt1; TNT; TNTS; troponin T slow skeletal muscle type; troponin T slow type isoform sTnT1; troponin T slow type isoform sTnT2; troponin T type 1 (skeletal, slow); troponin T, slow skeletal muscle; troponin T1, skeletal, slow; troponin T1, slow skeletal type; troponin-T1, skeletal, slow; zgc:193831; zgc:193865; ANM; NEM5; STNT; TNT; TNTS
• Gene ID (Entrez): 7138
• Gene Symbol: TNNT1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: TnTs, Slow skeletal muscle troponin T, sTnT
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Cardiovascular Research
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: ANM, NEM5, STNT, TNT, TNTS
• Target: TNNT1
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min