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Rockland Immunochemicals Rabbit TrueBlot: Anti-Rabbit IgG HRP - 18-8816-33
Supplier: Rockland Immunochemicals 18881633
Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Rabbit IgG TrueBlot is provided as 1000X solution. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the IP. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For IP, Anti-Rat IgG beads, or Anti-Rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. Specific conditions for each protein and antibody combination should be specifically optimized by the end user.
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