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Sigma Aldrich Fine Chemicals Biosciences TrueGel3D Hydrogel Kits CD
Supplier: Sigma Aldrich Fine Chemicals Biosciences TRUE11KT
The TrueGel3D hydrogel pre-configured kit is used to prepare a chemically defined hydrogel with stiffness of ~400 Pa (shear modulus) that can be customized to match that of native cell environment. The TrueGel3D hydrogel is formed by crosslinking of RGD (arginylglycylaspartic acid) degradable polymer with CD cell-degradable crosslinker. The cells are encapsulated during crosslinking where they can adhere to the polymer through the RGD peptide and grow within the hydrogel. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala) which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1 MMP3 MMP7 and MMP9).Gel can be further dissolved by TrueGel3D Enzymatic Cell Recovery SOLUTIONon (TRUEENZ-500UL) for post-culture analysis including RT-PCR western blots and other molecular biological studies.
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