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Sigma Aldrich Fine Chemicals Biosciences TrueGel3D Hydrogel Kits SL
Supplier: Sigma Aldrich Fine Chemicals Biosciences TRUE71KT
The gel is formed by stable thioether bonds between thiol groups on CD cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in fAST-DEXTRAN. SLO-Dextran does not have sites for cell attachment but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step.The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala) which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1 MMP3 MMP7 and MMP9).TrueGel3D enzymatic cell recovery SOLUTIONon (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR western blots and other molecular biological studies.
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