Trypsin, 1-250 from Porcine Pancreas , MP Biomedicals, LLC

Description

Trypsin, 1-250 from Porcine Pancreas has an activity >250 USP u/mg. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.

• The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture
• The 1:250 designation indicates the tryptic activity based on standardized testing
• This ratio indicates that one part trypsin will digest 250 parts casein in the USP activity assay
• Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain
• Trypsin is used to re-suspend cells adherent to the cell culture dish wall during the process of harvesting cells
• Trypsin can also be used to dissociate dissected cells
• Trypsin can be used to break down casein in breast milk
• Trypsin is commonly used in biological research during proteomics experiments to digest proteins into peptides for mass spectrometry analysis
• Trypsin can also be used to dissolve blood clots in its microbial form and treat inflammation in its pancreatic form
• One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified

Specifications

Chemical Identifiers

• CAS: 9002-07-7
• Molecular Formula: C35H47N7O10
• Molecular Weight (g/mol): 725.80
• InChI Key: WGWZNYKOUXOZTC-UHFFFAOYNA-N
• Synonym: tryptase, trypsin, tryptase from human lung, tryptase from human lung, buffered aqueous solution, ≥5 units/mg protein, acetic acid; tert-butyl n-2s-1-2s-1-2s-5-diaminomethylideneamino-1-5-methyl-2-oxochromen-7-yl amino-1-oxopentan-2-yl amino-3-hydroxy-1-oxopropan-2-yl amino-1-oxo-3-phenylpropan-2-yl carbamate
• PubChem CID: 5311489

• Color: White
• pH: ∼8.0
• Packaging: Glass
• Quantity: 20 g