Recombinant Protein;25UG;Biotinylated Human TSLP (R127A, R130A), His,Avitag (TSP-H82E0) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 18.5 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP (R127A, R130A), His,Avitag (Cat. No. TSP-H82E0) with a linear range of 2-31 ng/mL (QC tested).;Immobilized Monoclonal Anti-Human TSLP Antibody, Human IgG2 (S257A) at 2 μg/mL (100 μL/well) can bind Biotinylated Human TSLP (R127A, R130A), His,Avitag (Cat. No. TSP-H82E0) with a linear range of 0.03-1 ng/mL (Routinely tested).