Invitrogen™ Human TWEAK (TNFSF12) ELISA Kit

Catalog No.	Quantity
50-274-1197	10 x 96 Tests

Catalog No. 50-274-1197 Supplier Invitrogen™ Supplier No. EHTNFSF12X10

Description

ELISA

The Human TWEAK (TNFSF12) ELISA quantitates Hu TWEAK in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TWEAK. Principle of the method The Human TWEAK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.

Specifications

• Accession Number: O43508
• Assay Range: 40-3000 pg/mL
• Assay Sensitivity: 40 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cytokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: APO3 ligand; APO3/DR3 ligand; APO3L; Dr3l; Dr3lg; LOW QUALITY PROTEIN: tumor necrosis factor ligand superfamily member 12; MGC129581; MGC20669; TNF superfamily member 12; TNF-related weak inducer of apoptosis; TNFSF12; TNLG4A; tumor necrosis factor (ligand) superfamily member 12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand 4A; tumor necrosis factor ligand superfamily member 12; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; tumor necrosis factor superfamily member 12; TWEAK; UNQ181/PRO207; zgc:153941APO3 ligand; APO3/DR3 ligand; APO3L; Dr3l; Dr3lg; LOW QUALITY PROTEIN: tumor necrosis factor ligand superfamily member 12; MGC129581; MGC20669; TNF superfamily member 12; TNF-related weak inducer of apoptosis; TNFSF12; TNLG4A; tumor necrosis factor (ligand) superfamily member 12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand 4A; tumor necrosis factor ligand superfamily member 12; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; tumor necrosis factor superfamily member 12; TWEAK; UNQ181/PRO207; zgc:153941; APO3L; DR3LG; TNLG4A; TWEAK
• Gene ID (Entrez): 8742
• Gene Symbol: Tnfsf12
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: APO3 ligand, TNF-related weak inducer of apoptosis, TWEAK
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: TWEAK
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min