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Rockland Immunochemicals U-251 Whole Cell Lysate 500µg
SDP

Supplier:  Rockland Immunochemicals W09001GY4

Encompass_Preferred

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool.  If dissociating conditions are desired, add reducing agent prior to heating.  The recommended loading volume per lane is 10-20 µL depending on the size format of your gel.

Catalog No. 50-202-4107


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