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Eurofins Discoverx PathHunter U2OS NF-KB Reporter Cell Line, 2 vials. (93-1157C3)

Catalog No. 502002272
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The PathHunter Signaling Pathway Reporter Assays are stable clonal cell lines that monitor the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. PathHunter U2OS NF-kB Reporter Cell Line is engineered to express a synthetic DNA reporter construct, comprising a NFkB-specific transcription factor response element that drives expression of eProLabel-tagged reporter protein, and takes advantage of endogenous receptors. The activated signaling pathway induces expression of the eProLabel-tagged reporter protein, which is measured by addition of the detection reagent containing EA, resulting in complementation of the two enzyme fragments, and production of an active enzyme that hydrolyzes the substrate to generate a chemiluminescent signal. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.

Catalog No. 50-200-2272 Supplier Eurofins Discoverx Supplier No. 931157C3
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The PathHunter Signaling Pathway Reporter Assays are stable clonal cell lines that monitor the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. PathHunter U2OS NF-kB Reporter Cell Line is engineered to express a synthetic DNA reporter construct, comprising a NFkB-specific transcription factor response element that drives expression of eProLabel-tagged reporter protein, and takes advantage of endogenous receptors. The activated signaling pathway induces expression of the eProLabel-tagged reporter protein, which is measured by addition of the detection reagent containing EA, resulting in complementation of the two enzyme fragments, and production of an active enzyme that hydrolyzes the substrate to generate a chemiluminescent signal. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.

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