Invitrogen™ Human ULK1, GST Tag Recombinant Protein

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Supplier:  Invitrogen™ PV6430

Catalog No. PV6430

Description

The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Specifications

• For Use With (Application): Kinase Assay
• Quantity: 10μg
• Protein Length: aa 1-649
• Recombinant: Recombinant
• Content And Storage: Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Kinase Group: Serine/Threonine Kinase
• Expression System: Baculovirus
• Protein Form: Recombinant, Fragment
• Protein Subtype: Serine/Threonine Kinases

• Molecular Weight (g/mol): 96.1 kDa
• Gene Alias: ATG1, UNC51, Unc51.1, FLJ38455
• Species: Human
• Protein Tag: GST Tag
• Assay Entry: Biochemical binding/kinetic
• Target Entry: ULK1
• Protein Family: Kinases & Inhibitors
• Form: Liquid