Invitrogen™ Human ULK3, His Tag Recombinant Protein

Catalog No.	Quantity
PV6438	1 mg
PV6437	100 μg
PV6436	10 μg

Catalog No. PV6438 Supplier Invitrogen™ Supplier No. PV6438

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Full-length recombinant human ULK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

ULK3 or unc-51-like kinase 3 is a member of the serine/threonine kinase family that is involved in the SHH signaling pathway as a positive regulator of GLI proteins. ULK3 enhances endogenous and over-expressed GLI transcriptional activity in cultured cells and alters its subcellular localization. Furthermore, ULK3 phosphorylates GLI proteins in vitro. ULK3 is widely expressed and its expression is higher in a number of tissues where SHH signaling is known to be active, again suggesting that ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.

Specifications

• Accession Number: Q6PHR2
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: PBS with 150mM Imidazole, 0.1mM PMSF, 25% glycerol, 0.2mM DTT and no preservative; pH 7
• Gene ID (Entrez): 25989
• Molecular Weight (g/mol): 54.3 kDa
• Name: Human ULK3, His Tag
• pH Range: 7
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 1200015E14Rik; DKFZP434C131; EC:2.7.11.1; FLJ90566; Serine/threonine-protein kinase ULK3; ULK3; unc-51 like kinase 3; unc-51-like kinase 3
• Common Name: ULK3
• Gene Symbol: ULK3
• Protein Length: aa 44-end
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: 44-end
• Content And Storage: Storage Buffer: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Kinase Group: Serine/Threonine Kinase
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant
• Form: Liquid