Diagnocine LLC UltraRIPA kit for Lipid Raft

Supplier:  Diagnocine LLC FNKF015

RIPA buffer is one of the most useful buffers for protein extraction. RIPA buffer maintains most native structures of proteins and the extracted proteins can be applied to various applications. However, RIPA buffer is not sufficient to extract membrane proteins and membrane-associated proteins concentrated in lipid raft. Lipid raft is a highly specialized microdomain on the lipid bilayer which contains specialized lipids, cholesterol and functional proteins. These lipid rafts are also called “Detergent Resistant Membrane (DRM)”, as lipid raft-enriched proteins are usually insoluble by mild detergent buffers such as 1% Triton X-100 and RIPA buffer. Consequently, it was difficult to analyze functions of lipid raft-enriched proteins extracted with RIPA buffer.

Catalog No. NC1635811