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New England Biolabs, Inc. Universal miRNA Cloning Linker – 5 µg

Supplier: New England Biolabs, Inc. S1315S

Linker/RNA Ligation Protocol: The Universal miRNA cloning linker has a 3' blocking group (amine) to prevent self ligation, circularization and ligation to RNA at the 5' end. It also contains the BanI restriction site compatible with the miRNA cloning protocol used by Bartel. For ligation, resuspend the lyophilized oligonucleotide in RNAse-free water to 100 M stock concentration. (A resuspension volume of 8.6 µl will give a 0.58ug/l or 100 µM stock solution). Ligations are carried out with T4 RNA Ligase I in the absence of ATP. Approximately 1 ug is typically used per reaction.
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