Invitrogen™ Human uPa/PRAP1 ELISA Kit

Catalog No.	Quantity
EHPRAP1X10	10 x 96 Tests

Catalog No. EHPRAP1X10 Supplier Invitrogen™ Supplier No. EHPRAP1X10

Description

ELISA

The Human uPa/PRAP1 ELISA quantitates Hu uPa in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu uPa. Principle of the method The Human uPa solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signaling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening. PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.

Specifications

• Accession Number: Q96NZ9
• Assay Range: 10-3200 pg/mL
• Assay Sensitivity: 10 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: epididymis tissue protein Li 178; PRAP1; Pregnancy-specific uterine protein; PRO1195; proline rich acidic protein 1; proline-rich acidic protein 1; UNQ608/PRO1195; Upa; uterine-specific proline-rich acidic proteinepididymis tissue protein Li 178; PRAP1; Pregnancy-specific uterine protein; PRO1195; proline rich acidic protein 1; proline-rich acidic protein 1; UNQ608/PRO1195; Upa; uterine-specific proline-rich acidic protein; PRO1195; UPA
• Gene ID (Entrez): 118471
• Gene Symbol: PRAP1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Epididymis tissue protein Li 178, Uterine-specific proline-rich acidic protein
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: PRAP1
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min