Invitrogen™ Human VCAM-1 ELISA Kit

Catalog No.	Quantity
KHT0601	96 Tests

Catalog No. KHT0601 Supplier Invitrogen™ Supplier No. KHT0601

Description

ELISA

The Human Vascular Cell Adhesion Molecule 1 (Hu VCAM-1) ELISA quantitates Hu VCAM-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VCAM-1. Principle of the method The Human VCAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

Specifications

• Accession Number: P19320
• Assay Range: 0.59-75 ng/mL
• Assay Sensitivity: <0.5 ng/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: adhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1Badhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1B; CD106; INCAM-100
• Gene ID (Entrez): 7412
• Gene Symbol: VCAM1
• Incubation Time: 3 hr.
• Interassay CV: 6.4%
• Intraassay CV: 6.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Marker Gene: VCAM-1
• Protein Aliases: V-CAM 1, VCAM-1, INCAM-100
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: VCAM-1 (CD106)
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr