Thermo Scientific™ ViewRNA™ HC Screening Assay

Description

The ViewRNA HC Screening assay is an in situ hybridization method for visualization of up to 4 target RNAs, at single transcript detection level, simultaneously within individual cells. Dual detection with protein also possible, by optimizing experimental conditions.

The ViewRNA HC Screening assay is an in situ hybridization method for visualization of up to 4 target RNAs, at single transcript detection level, simultaneously within individual cells. Dual detection with protein also possible, by optimizing experimental conditions. For flexibility, ViewRNA HC Screening Assay provided in three modules (each sold separately): ViewRNA HC Screening Assay Kit, ViewRNA HC Screening Signal Amplification Kit, and ViewRNA Probe Sets. All there modules are needed to perform ViewRNA HC Screening Assay. Note: ViewRNA HC Screening Assay Kit and ViewRNA HC Screening Signal Amplification Kit are made to order kits. Please allow 4-6 weeks for shipment after placing order.

With ViewRNA Tissue assays, you can achieve

• Detects up to 4 RNA targets at single transcription level in cell culture cells in an automated format for 96- or 384-well plates
• Utilizes bright Alexa Flour 488, Alexa Flour 546, Alexa Flour 947, and/or Alexa Flour 750.
• Dual detection with proteins possible, by optimizing experimental conditions.

The ViewRNA assay is a novel RNA in situ hybridization solution, based on patent-pending Probe Set design and proprietary signal amplification technology. ViewRNA assays offers single copy RNA sensitivity in individual cells in a multiplex assay format. Signal amplification is predicated on specific hybridization of adjacent Probe Set oligonucleotides to a target RNA, resulting in excellent signal-to-noise ratios. Independent but compatible signal amplification systems enable simultaneous detection of up to 4 target RNAs in a single assay.

The ViewRNA HC Screening Assay is comprised of 3 modules (each sold separately):

• ViewRNA HC Screening Assay Kit
• ViewRNA HC Screening Signal Amplification Kit
• ViewRNA Probe Sets. Probes can be ordered from https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-cell-assays.html

How it Works

Step 1: Prepare Sample. Adherent cells on a solid surface are fixed and permeabilized. Optional perform protein detection, by following immunocytochemistry protocol.

Step 2: Hybridize Probe Sets. Gene-specific Probe Sets hybridize to target mRNAs.

Step 3: Amplify Signal. Independent but compatible signal amplification systems enable simultaneous detection of multiple RNAs in a single assay. Distinct sets of PreAmp, Amp, and Label Probe molecules are used to detect different target RNAs.

Step 4: Image. Target mRNAs are visualized using high content imaging systems or standard epifluorescence microscope.

Components of ViewRNA HC Screening Assay Kits.

Store following components at 2°C to 8°C

• Pretease QS: Enzyme in aqueous buffered solution
• Probe Set Diluent QS: Aqueous solution containing formamide and detergent
• Amplifier Diluent QS Aqueous solution containing formamide and detergent
• Label Probe Diluent QS Aqueous solution containing detergent

Store following components at room temperature.

• Protease Stop Buffer Aqueous buffered solution
• Storage Buffer Aqueous buffered solution
• Detergent Solution: Aqueous buffered solution containing detergent
• Wash Buffer Component 1 (Wash Comp 1): Aqueous solution containing detergent
• Wash Buffer Component 2 (Wash Comp 2): Aqueous buffered solution
• Pre-Hybridization Buffer (PreHyb Buffer): Aqueous buffered solution

Components of ViewRNA HC Screening Signal Amplification Kits:

Store following components at -20°C.

• PreAmplifier (PreAmp type 4, 6, 8, or 10): DNA in aqueous buffered solution.
• Amplifier (Amp type 4, 6, 8, or 10): DNA in aqueous buffered solution.
• Label Probe (LP type 4-Alexa Flour 488, LP type 1-Alexa Flour 546, LP type 6-Alexa Flour 647, LP type 10-Alexa Flour 750): Fluorescent dye-conjugated oligonucleotides in aqueous buffered solution.

Note:

• 1-plex kits only have type 1 reagents
• 2-plex kits have type 1 and 6 reagents
• 3-plex kits have type 1, 4 and 6 reagents
• 4-plex kits have type 1, 4, 6 and 10 reagents

Specifications

• Detection Method: Fluorescence
• Format: Kit
• Label or Dye: Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 647, Alexa Fluor 750
• Final Product Type: Probes (Labeled DNA), Probes (Labeled RNA)
• Sample Type: Cell Culture Cells
• Labeling Target: RNA (General)
• Excitation/Emission: 556/573, 650/665, 496/519, 749/775 nm
• For Use With (Equipment): Fluorescence Microscope, or High Content Analyzer
• Labeling Method: ViewRNA Type 1, 4, 6, and 10
• Product Line: ViewRNA™
• Product Type: HC Screen. Signal Amp., 4 Plex
• Quantity: 100 Plates